![]() ![]() ![]() Successful induction of conjugation under laboratory conditions has been reported in only a few cases (e.g., Czurda, 1930 Ikegaya et al., 2012 Zwirn et al., 2013 El-Sheekh et al., 2018 Takano et al., 2019 Permann et al., 2021b Pfeifer et al., 2022). (b) Details of part of the ML tree (a) showing Clades III-VI. Details of "Desmidiales" are shown in Supplementary Fig. (a) Overview of the ML tree based on rbcL and atpB concatenated dataset. " may belong to Temnogyra or Sirogonium because of lack of sexual reproduction characteristics. Note that OTUs of I-VII correspond to those of Clades I-VII of Stancheva et al. OTU designations in boldface represent 52 rbcL-types from Japan (Supplementary Table S1). Asterisk represents 100% bootstrap values by ML method or 1.00 posterior probability by BI. Numbers at left and right sides above branches are bootstrap values (50% or more) by ML analysis and posterior probabilities (0.90 or more) by Bayesian inference (BI), respectively. Branch lengths are proportional to the evolutionary distances indicated by scale bar above the tree or subtree in each figure. Maximum likelihood (ML) tree based on the combined data set from rbcL and atpB genes of 138 operational taxonomic units (OTUs) (zygnematophycean algae) and five outgroup OTUs (Coleochaete) (Supplementary Tables S1, S4) using RAxML 26. Thus, separation of Temnogyra from Spirogyra may be untenable, much as the separation of Sirogonium from Spirogyra is not supported by molecular analyses. Our phylogenetic analysis demonstrated that the two Temnogyra species are included in a large clade comprising many species of Spirogyra. Two of the thirteen species were assignable to a related but taxonomically uncertain genus, Temnogyra, based on the unique characteristics of sexual reproduction. We induced conjugation or aplanospore formation under controlled laboratory conditions in 15 of the 52 strains, which allowed us to identify 13 species. We tested the modified procedure on 52 newly established and genetically different strains collected from diverse localities in Japan. We developed an identification procedure based on an improved methodology for inducing sexual conjugation in laboratory-cultivated filaments. ![]() Species assignment is often difficult because identification is based on the characteristics of sexual reproduction in wild-collected samples and spores produced in the field or laboratory culture. The genus Spirogyra is abundant in freshwater habitats worldwide, and comprises approximately 380 species. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |